Transport and accumulation of triazenoimidazoles by L1210 cells.

Amethopterin Transport in Ehrlich Ascites Carcinoma and L1210 Cells1

Accumulation of amethopterin by L1210 and Ehrlich ascites cells in miro was mediated by a slow, temperature-sensitive, and partly saturable process. The structural specificity of the proc ess could be measured by competition studies; differences were found between the two cell lines. At physiologic drug levels, i.e., those found in vivo during drug therapy, concentrativi uptake of amethopterin ...

Amethopterin transport in Ehrlich ascites carcinoma and L1210 cells.

Accumulation of amethopterin by L1210 and Ehrlich ascites cells in miro was mediated by a slow, temperature-sensitive, and partly saturable process. The structural specificity of the proc ess could be measured by competition studies; differences were found between the two cell lines. At physiologic drug levels, i.e., those found in vivo during drug therapy, concentrativi uptake of amethopterin ...

Effects of nucleoside transport inhibitors on the salvage and toxicity of adenosine and deoxyadenosine in L1210 and P388 mouse leukemia cells.

Incubation of deoxycoformycin-treated L1210 leukemia cells with dipyridamole or nitrobenzylthioinosine, inhibitors of nucleoside transport, enhanced the long-term incorporation of 2'-deoxyadenosine and adenosine into the nucleotide pool and the toxicity of 2'-deoxyadenosine for the cells. In contrast, 2'-deoxyadenosine uptake in deoxycoformycin-treated P388 leukemia cells, which was about 10 ti...

Sodium-dependent and equilibrative nucleoside transport systems in L1210 mouse leukemia cells: effect of inhibitors of equilibrative systems on the content and retention of nucleosides.

The presence of 10 microM dipyridamole in incubation media of L1210/C2 cells decreased initial rates of zero-trans influx of formycin B (FB, 50 microM), a poorly metabolized inosine analogue, from 4.84 pmol/microliters cell water/s to 0.87 pmol/microliter cell water/s. However, after a 5-min interval of uptake, free FB levels in dipyridamole-treated cells were 165 pmol/microliters cell water, 2...

Transport energetics of the folic acid analogue, methotrexate, in L1210 leukemia cells. Enhanced accumulation by metabolic inhibitors.